先遍历每一行，判断checkBox是否选中，再获取选中行的主键Id 删除就行了 ,,,
foreach(DatalistRow rowview in Datalist.Rows) //遍历Datalist中的每一行
{
//假设你的Datalist中的复选框放在第一列，id是“CheckBox1”
CheckBox check = （CheckBox）rowview.Cells[0].FindControl("CheckBox1");//找到了checkbox控件
if(check.Checked)//如果被选中
{
...
//假设你把每一行的id放在第二列
string myid = rowview.Cells[1].text;//这就是所在行的id，赋值给了myid
....//作删除操作
}
}
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